Screening and Identification of Antibiotic Resistant Gene <i>int1</i> in Coliforms Isolated From Drinking Water

Antibiotic-resistance genes carried by coliforms in drinking water is a concerning issue for public health Bangladesh. This research was out to identify and understand the importance of int1 gene spread resistance bacterial antibiotics through consumption contaminated water. A total 31 samples were collected from restaurants ( n = 18), center 9), residences 4) located Chattogram City, The isolation identification performed on selective media with combination biochemical molecular analysis. PCR amplification LacZ, uidA coliform fecal antibiotic resistant gene, respectively. Antimicrobial susceptibility test according Kirby-Bauer disk diffusion method McFarland standard against three including co-trimoxazole, ciprofloxacin, ampicillin. Of samples, detected within 32% 10) nine one sample residence. But no center. Among identified coliforms, prevalence 60% 6) 40% 4), relatively. All isolates containing microbial-resistance ampicillin.This study shows that consumed different Chattogram, Bangladesh antibiotic-resistant bearing not only increase risk water-borne disease, but also may be major cause transmission this part